Anticorps Polyclonal de lapin anti-STXBP6

STXBP6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 10976-4-AP

Synonymes

Amisyn, Syntaxin-binding protein 6, STXBP 6, HSPC156

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Galerie de données de validation

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  WB analysis of mouse brain using 10976-4-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10976-4-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10976-4-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10976-4-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10976-4-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10976-4-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10976-4-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10976-4-AP (STXBP6 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules BxPC-3, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10976-4-AP cible STXBP6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STXBP6 Protéine recombinante Ag1427
• Nom complet: syntaxin binding protein 6 (amisyn)
• Masse moléculaire calculée: 25 kDa or 45 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC009499
• Symbole du gène: STXBP6
• Identification du gène (NCBI): 29091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,rat	WB	BMC Med; A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression; Authors - Yu Qian

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