Anticorps Monoclonal anti-SULT1A1

• Phare
• Validé par KD/KO

SULT1A1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

10H6F6

N° de cat : 60076-1-Ig

Synonymes

EC:2.8.2.1, HAST1/HAST2, OK/SW-cl.88, P PST, P PST 1

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Galerie de données de validation

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  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60076-1-Ig

  WB result of SULT1A1 antibody (60076-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SULT1A1 transfected Jurkat cells.

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  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 60076-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 60076-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60076-1-Ig (SULT1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules U2OS, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60076-1-Ig cible SULT1A1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SULT1A1 Protéine recombinante Ag4611
• Nom complet: sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000923
• Symbole du gène: SULT1A1
• Identification du gène (NCBI): 6817
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SULT1A1, also named as ST1A1, P-PST 1, ST1A3, Ts-PST, P-PST 1, STP and STP1, belongs to the sulfotransferase 1 family. It catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. SULT1A1 is also responsible for the sulfation and activation of minoxidil. It mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity.

Publications

Species	Application	Title
rat	WB	J Chromatogr B Analyt Technol Biomed Life Sci; Liquid chromatography coupled with high resolution mass spectrometry reveals the inhibitory effects of Huangkuisiwu formula on biosynthesis of protein-binding uremic toxins in rats with chronic kidney disease; Authors - Jianping Li

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