Anticorps Recombinant de lapin anti-SULT1B1

SULT1B1 Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240119C12

N° de cat : 83228-1-PBS

Synonymes

240119C12, EC:2.8.2.1, ST1B1, ST1B2, SULT1B2

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Galerie de données de validation

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  WB analysis of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 83228-1-RR (SULT1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83228-1-RR (same clone as 83228-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SULT1B1 Recombinant antibody (83228-1-RR, Clone:240119C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00184-1

  Cytometric bead array standard curve of MP00184-1, SULT1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83228-1-PBS. Detection antibody: 83228-2-PBS. Standard: Ag9009. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83228-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83228-1-PBS against Human SULT1B1 was performed. The affinity constant is 5.19 nM.

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  Affinity and Kinetic Characterization of 83228-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83228-1-RR against Human SULT1B1 were performed. The affinity constant is 63.8 nM.

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Informations sur le produit

83228-1-PBS cible SULT1B1 dans les applications de WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SULT1B1 Protéine recombinante Ag9009
• Nom complet: sulfotransferase family, cytosolic, 1B, member 1
• Masse moléculaire calculée: 296 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC010895
• Symbole du gène: SULT1B1
• Identification du gène (NCBI): 27284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

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