Anticorps Polyclonal de lapin anti-SUMO1

SUMO1 Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 10329-1-AP

Synonymes

DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1

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Galerie de données de validation

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  WB analysis using 10329-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10329-1-AP (SUMO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10329-1-AP

  IP result of anti-SUMO1(IP:10329-1-AP, 4ug; Detection:10329-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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  IHC staining of human testis using 10329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10329-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10329-1-AP (SUMO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 10329-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SUMO1 antibody (10329-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 10329-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human SUMO1 (10329-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules A549
• Résultats positifs en cytométrie: cellules A549,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

10329-1-AP cible SUMO1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUMO1 Protéine recombinante Ag0414
• Nom complet: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 10-12 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: BC006462
• Symbole du gène: SUMO1
• Identification du gène (NCBI): 7341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Small ubiquitin-related modifier 1 (SUMO1) belongs to the ubiquitin family and the SUMO subfamily as it contains 1 ubiquitin-like domain and is also 18% identical to ubiquitin (PMID: 9654451).

1. What is the molecular weight of SUMO1?

The molecular weight of SUMO1 is 12 kDa.

2. What is the cellular localization of SUMO1?

SUMO1 is located in the nuclear membrane and is recruited by BCL11A to the nuclear body (PMID: 18681895).

3. What modifications is SUMO1 subjected to?

SUMO1 function occurs after the cleavage of its precursor form by SENP1 or SENP2 (PMID: 27576863). Polymeric SUMO1 chains also undergo polyubiquitination by RNF4 (PMID: 18408734).

4. What is the function of SUMO1?

SUMO1 post-translationally modifies many proteins that have roles in an array of processes including transcriptional regulation, chromatin structure, and DNA repair. SUMO1 can covalently attach itself to protein as a monomer or a lysine-linked polymer via an isopeptide bond (PMID:11124955). This SUMO modification can regulate the localization and activity of the affected proteins.

5. What is SUMO1's involvement in disease?

Defects in SUMO1 as a result of a chromosomal translocation are associated with non-syndromic orofacial cleft type 10, or non-syndromic cleft lip, and are associated with cleft palate in two-thirds of cases (PMID: 18983974).

Publications

Species	Application	Title
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Theranostics; Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.; Authors - Shuai Huang
human	WB	Anal Chem; Sensitive detection of intracellular sumoylation via SNAP tag-mediated translation and RNA polymerase-based amplification.; Authors - Yong Yang
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
human	WB	J Mol Cell Biol; SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.; Authors - Xiaoli Xu
mouse	IP	Int J Mol Med; SUMO2 modification of Aurora B and its impact on follicular development and atresia in the mouse ovary.; Authors - Jing Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zoe (Verified Customer) (10-08-2021)

Hadn’t previously had much luck with antibodies on my Tricky construct so was great to use this one!

• Applications: Western Blot

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