Anticorps Monoclonal anti-SUMO1

SUMO1 Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B5B5

N° de cat : CL488-67559

Synonymes

Small ubiquitin related modifier 1, Sentrin, OK/SW-cl.43, GMP1, GAP-modifying protein 1

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Galerie de données de validation

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  IF Staining of MCF-7 using CL488-67559

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 SUMO1 antibody (CL488-67559, Clone: 3B5B5 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67559 cible SUMO1 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SUMO1 Protéine recombinante Ag29402
• Nom complet: SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 15-100 kDa
• Numéro d’acquisition GenBank: BC006462
• Symbole du gène: SUMO1
• Identification du gène (NCBI): 7341
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.This antibody can detect endogenous levels of SUMOylated proteins (e.g. SUMO-1-RanGAP at 80-90 kD).

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