Anticorps Recombinant de lapin anti-SUMO2/3

SUMO2/3 Recombinant Antibody for IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241156E1

N° de cat : 84017-5-PBS

Synonymes

SUMO3, SUMO 2, SMT3H1, SMT3A, SMT3 homolog 1

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Galerie de données de validation

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  IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SUMO3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84017-5-RR (same clone as 84017-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUMO2/3 antibody (84017-5-RR, Clone: 241156E1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84017-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84017-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84017-5-RR against Human SUMO2/3 were performed. The affinity constant is 6.81 nM.

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Informations sur le produit

84017-5-PBS cible SUMO2/3 dans les applications de IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SUMO2/3 Protéine recombinante Ag1135
• Nom complet: SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• Masse moléculaire calculée: 12 kDa
• Numéro d’acquisition GenBank: BC008420
• Symbole du gène: SUMO3
• Identification du gène (NCBI): 6612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

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