Anticorps Polyclonal de lapin anti-SUN2

• Phare
• Validé par KD/KO

SUN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 27556-1-AP

Synonymes

UNC84B, FRIGG, KIAA0668, Protein unc-84 homolog B, Rab5-interacting protein

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Galerie de données de validation

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  WB analysis using 27556-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27556-1-AP

  WB result of SUN2 antibody (27556-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUN2 transfected HeLa cells.

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  WB analysis of mouse testis using 27556-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 27556-1-AP (SUN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 27556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27556-1-AP (SUN2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27556-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUN2 antibody (27556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

27556-1-AP cible SUN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUN2 Protéine recombinante Ag16077
• Nom complet: unc-84 homolog B (C. elegans)
• Masse moléculaire calculée: 717 aa, 80 kDa
• Poids moléculaire observé: 80-85 kDa
• Numéro d’acquisition GenBank: BC111717
• Symbole du gène: SUN2
• Identification du gène (NCBI): 25777
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).

Publications

Species	Application	Title
human	WB	Cancer Cell; Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite; Authors - Yihong Li
human	WB	Virology; Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection; Authors - Chao Yang
human	WB,IF	Nat Commun; Nuclear membrane protein SUN2 promotes replication of flaviviruses through modulating cytoskeleton reorganization mediated by NS1; Authors - Yanxia Huang ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nuo (Verified Customer) (01-28-2023)

There are several non specific bands nearby.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

SUN2 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27556-1-AP)

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