Anticorps Monoclonal anti-SUPT16H

• Phare
• Validé par KD/KO

SUPT16H Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1E4C4

N° de cat : 68243-1-Ig

Synonymes

CDC68, FACT 140 kDa subunit, FACT complex subunit SPT16, FACT140, FACTP140, FLJ10857, FLJ14010, FLJ34357, hSPT16, SPT16/CDC68, SUPT16H

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Galerie de données de validation

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  WB analysis of MCF-7 using 68243-1-Ig

  WB result of SUPT16H antibody (68243-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SUPT16H transfected MCF-7 cells.

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  WB analysis using 68243-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68243-1-Ig (SUPT16H antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  IF Staining of HeLa using 68243-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPT16H antibody (68243-1-Ig, Clone: 1E4C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68243-1-Ig cible SUPT16H dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SUPT16H Protéine recombinante Ag29459
• Nom complet: suppressor of Ty 16 homolog (S. cerevisiae)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: NM_007192
• Symbole du gène: SUPT16H
• Identification du gène (NCBI): 11198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SUPT16H, also named as FACT140, FACTP140, SPT16 and CDC68, belongs to the peptidase M24 family and SPT16 subfamily. SUPT16H is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). It also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.