Anticorps Polyclonal de lapin anti-SUPT6H

SUPT6H Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23073-1-PBS

Synonymes

SPT6H, SPT6, KIAA0162, hSPT6, Histone chaperone suppressor of Ty6

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Galerie de données de validation

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  WB analysis of HeLa using 23073-1-AP (same clone as 23073-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23073-1-AP (same clone as 23073-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23073-1-AP (same clone as 23073-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 23073-1-AP (same clone as 23073-1-PBS)

  IP result of anti-SUPT6H (IP:23073-1-AP, 4ug; Detection:23073-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 23073-1-AP (same clone as 23073-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23073-1-AP (SUPT6H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23073-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23073-1-PBS cible SUPT6H dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUPT6H Protéine recombinante Ag19426
• Nom complet: suppressor of Ty 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 1726 aa, 199 kDa
• Poids moléculaire observé: 230 kDa
• Numéro d’acquisition GenBank: BC136524
• Symbole du gène: SUPT6H
• Identification du gène (NCBI): 6830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and directly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

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