Anticorps Recombinant de lapin anti-SUPV3L1

SUPV3L1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250855G8

N° de cat : 86706-1-PBS

Synonymes

ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1

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Galerie de données de validation

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  WB analysis using 86706-1-RR (same clone as 86706-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 86706-1-RR (same clone as 86706-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 86706-1-RR (SUPV3L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86706-1-RR (same clone as 86706-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUPV3L1 antibody (86706-1-RR, Clone: 250855G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86706-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02602-1

  The mean SUPV3L1 concentration was determined to be 102.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 171.2 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02602-1

  Cytometric bead array standard curve of MP02602-1, SUPV3L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86706-1-PBS. Detection antibody: 86706-3-PBS. Standard: Ag3542. Range: 1.562-200 ng/mL.

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Informations sur le produit

86706-1-PBS cible SUPV3L1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SUPV3L1 Protéine recombinante Ag3542
• Nom complet: suppressor of var1, 3-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 786 aa, 88 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC036112
• Symbole du gène: SUPV3L1
• Identification du gène (NCBI): 6832
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

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