Anticorps Polyclonal de lapin anti-SURF1

• Phare
• Validé par KD/KO

SURF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15379-1-PBS

Synonymes

SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

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Galerie de données de validation

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  WB analysis using 15379-1-AP (same clone as 15379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15379-1-PBS cible SURF1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SURF1 Protéine recombinante Ag3959
• Nom complet: surfeit 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-31 kDa
• Numéro d’acquisition GenBank: BC028314
• Symbole du gène: SURF1
• Identification du gène (NCBI): 6834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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