Anticorps Recombinant de lapin anti-SURF1

• Phare
• Validé par KD/KO

SURF1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230210E12

N° de cat : 83052-6-PBS

Synonymes

230210E12, SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

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Galerie de données de validation

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  WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

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  WB analysis using 83052-6-RR (same clone as 83052-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83052-6-RR (same clone as 83052-6-PBS)

  WB result of SURF1 antibody (83052-6-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SURF1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83052-6-RR (same clone as 83052-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83052-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83052-6-RR against Human SURF1 were performed. The affinity constant is 0.509 nM.

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Informations sur le produit

83052-6-PBS cible SURF1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SURF1 Protéine recombinante Ag3959
• Nom complet: surfeit 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-31 kDa
• Numéro d’acquisition GenBank: BC028314
• Symbole du gène: SURF1
• Identification du gène (NCBI): 6834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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