Anticorps Recombinant de lapin anti-SURF1

SURF1 Recombinant Antibody for IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230210D5

N° de cat : 83052-7-PBS

Synonymes

230210D5, SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

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Galerie de données de validation

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  IP experiment of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  IP result of anti-SURF1 (IP:83052-7-RR, 4ug; Detection:83052-7-RR 1:2000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 83052-7-RR (same clone as 83052-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83052-7-RR (SURF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83052-7-RR (same clone as 83052-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SURF1 antibody (83052-7-RR, Clone: 230210D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83052-7-RR (same clone as 83052-7-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-7-RR, Clone:230210D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83052-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83052-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83052-7-RR against Human SURF1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83052-7-PBS cible SURF1 dans les applications de IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SURF1 Protéine recombinante Ag3959
• Nom complet: surfeit 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30-31 kDa
• Numéro d’acquisition GenBank: BC028314
• Symbole du gène: SURF1
• Identification du gène (NCBI): 6834
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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