Anticorps Polyclonal de lapin anti-SURVIVIN

• Phare
• Validé par KD/KO

SURVIVIN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

133

Avis (2)

N° de cat : 10508-1-AP

Synonymes

API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, EPR 1, IAP4, SURVIVIN

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Galerie de données de validation

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  WB analysis of Jurkat using 10508-1-AP

  WB result of Survivin antibody (10508-1-AP, 1:5000) with si-control and si-Survivin transfected Jurkat cells..

• 

  WB analysis using 10508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10508-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10508-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U2OS using 10508-1-AP

  IP result of anti-SURVIVIN(IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:1000) with U2OS cells lysate 440 ug.

• 

  IP experiment of Jurkat using 10508-1-AP

  IP Result of anti-SURVIVIN (IP:10508-1-AP, 4ug; Detection:10508-1-AP 1:300) with Jurkat cells lysate 2120ug.

• 

  IHC staining of human colon cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10508-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10508-1-AP (SURVIVIN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10508-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SURVIVIN antibody (10508-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 10508-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SURVIVIN (10508-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-3, cellules Ramos
• Résultats positifs en IP: cellules U2OS, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'estomac humain, tissu de cancer du sein humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 120 publications below
• CoIP: See 1 publications below

Informations sur le produit

10508-1-AP cible SURVIVIN dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SURVIVIN Protéine recombinante Ag0788
• Nom complet: baculoviral IAP repeat-containing 5
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC008718
• Symbole du gène: BIRC5
• Identification du gène (NCBI): 332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Publications

Species	Application	Title
human	WB	Biomaterials; PMID: 29028548; Authors - Yudong Song
human,mouse	WB	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	WB	Autophagy; Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells.; Authors - Xuejiao Zhao
human	WB	Circ Res; Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.; Authors - Emanuele Cotroneo
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Int J Biol Sci; Polyphyllin VII induces CTC anoikis to inhibit lung cancer metastasis through EGFR pathway regulation; Authors - Zujun Que

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Susmita (Verified Customer) (08-21-2020)

Giving good result and crisp band in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Pancreatic cell line

SURVIVIN Antibody Western Blot validation (1:1000 dilution) in Pancreatic cell line (Cat no:10508-1-AP)

FH

Iram (Verified Customer) (08-14-2020)

We got very sharp and clean band of survivin with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human colon cancer

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