• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-SURVIVIN

SURVIVIN Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

3B9H7

N° de cat : 66495-1-Ig

Synonymes

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5



Applications testées

Résultats positifs en WBcellules HEK-293, cellules A431, cellules HeLa, cellules Jurkat, cellules K-562, cellules Raji, cellules U-937
Résultats positifs en IHCtissu d'amygdalite humain, tissu de cancer de la peau humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochimie (IHC)IHC : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66495-1-Ig cible SURVIVIN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène SURVIVIN Protéine recombinante Ag20958
Nom complet baculoviral IAP repeat-containing 5
Masse moléculaire calculée 16 kDa
Poids moléculaire observé 18 kDa
Numéro d’acquisition GenBankBC008718
Symbole du gène Survivin
Identification du gène (NCBI) 332
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

Protocole

Product Specific Protocols
WB protocol for SURVIVIN antibody 66495-1-IgDownload protocol
IHC protocol for SURVIVIN antibody 66495-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

Phytomedicine

Astragaloside Ⅳ negatively regulates Gpr97-TPL2 signaling to protect against hyperhomocysteine-exacerbated sepsis associated acute kidney injury

Authors - Jingge Xu
humanWB

Bioeng Transl Med

Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.

Authors - Fabiao Hu
humanWB

Exp Mol Med

m 6 A-mediated upregulation of AC008 promotes osteoarthritis progression through the miR-328-3p‒AQP1/ANKH axis

Authors - Jiashu Yang
humanWB,IF

Acta Biochim Biophys Sin (Shanghai)

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.

Authors - Junquan Gu
  • KD Validated
mouseWB

Front Oncol

Synergistic effects of nab-PTX and anti-PD-1 antibody combination against lung cancer by regulating the Pi3K/AKT pathway through the Serpinc1 gene

Authors - Jun Zhang
humanWB

Front Pharmacol

Analysis of regulating activities of 5'-epiequisetin on proliferation, apoptosis, and migration of prostate cancer cells in vitro and in vivo

Authors - Xueni Wang
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