Anticorps Monoclonal anti-SURVIVIN

• Phare
• Validé par KD/KO

SURVIVIN Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B9H7

N° de cat : 66495-1-PBS

Synonymes

BIRC5, IAP4, EPR 1, BIRC 5, Baculoviral IAP repeat-containing protein 5

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Galerie de données de validation

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  WB analysis using 66495-1-Ig (same clone as 66495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66495-1-Ig (same clone as 66495-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66495-1-Ig (same clone as 66495-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66495-1-Ig (SURVIVIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66495-1-Ig (same clone as 66495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66495-1-Ig (SURVIVIN antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66495-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66495-1-PBS cible SURVIVIN dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SURVIVIN Protéine recombinante Ag20958
• Nom complet: baculoviral IAP repeat-containing 5
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC008718
• Symbole du gène: Survivin
• Identification du gène (NCBI): 332
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin.

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