Anticorps Recombinant de lapin anti-SUSD2

SUSD2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242328A8

N° de cat : 84852-4-RR

Synonymes

242328A8, BK65A6.2, sushi domain containing 2

---

Galerie de données de validation

• 

  WB analysis of SK-BR-3 using 84852-4-RR

  SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 84852-4-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84852-4-RR (SUSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SK-BR-3 using 84852-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SK-BR-3 cells using SUSD2 antibody (84852-4-RR, Clone: 242328A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: SK-BR-3 cells, tissu placentaire humain
• Résultats positifs en IF/ICC: SK-BR-3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84852-4-RR cible SUSD2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SUSD2 Protéine recombinante Eg2627
• Nom complet: sushi domain containing 2
• Masse moléculaire calculée: 90 kDa
• Poids moléculaire observé: 60 kDa, 90 kDa, 110 kDa
• Numéro d’acquisition GenBank: NM_019601.3
• Symbole du gène: SUSD2
• Identification du gène (NCBI): 56241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sushi domain containing 2 (SUSD2) is a type I transmembrane protein that contains a somatomedin B, AMOP, von Willebrand factor type D and Sushi domains, which are frequently found in molecules associated with cell-cell and cell-matrix adhesion. SUSD2 is composed of 822 amino acids with a predicted molecular weight of 90.4 kDa (PMID: 31387209). Three specific SUSD2 bands were present, the glycosylated, full-length protein (~110 kDa), ~90 kDa band represents full-length SUSD2 with minimal glycosylation, and a strong ~60 kDa band, represents a cleaved product of SUSD2 at the GD-PH sequence in the endoplasmic reticulum (PMID:27775699, 32595828).

{{ptg:RelatedPrimaryAntibodies}}