Anticorps Polyclonal de lapin anti-SUV39H1

• Phare
• Validé par KD/KO

SUV39H1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10574-1-AP

Synonymes

H3 K9 HMTase 1, KMT1A, Lysine N methyltransferase 1A, MG44, Su(var)3 9 homolog 1, SUV39H, SUV39H1

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Galerie de données de validation

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  WB analysis using 10574-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10574-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10574-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 10574-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10574-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10574-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10574-1-AP

  IP Result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu d'intestin grêle de souris
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below

Informations sur le produit

10574-1-AP cible SUV39H1 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUV39H1 Protéine recombinante Ag0854
• Nom complet: suppressor of variegation 3-9 homolog 1 (Drosophila)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC006238
• Symbole du gène: SUV39H1
• Identification du gène (NCBI): 6839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.

Publications

Species	Application	Title
		Nucleic Acids Res; ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication; Authors - Huanzhou Xu
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang ; KO Validated
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Cell Death Dis; Dicer promotes tumorigenesis by translocating to nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells.; Authors - Wenlong Cheng
human	WB	Oncotarget; Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression.; Authors - Wenlong Cheng
human	WB	iScience; Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.; Authors - Jun Yamamoto