Anticorps Polyclonal de lapin anti-SUV39H1

• Phare
• Validé par KD/KO

SUV39H1 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10574-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10574-1-AP (same clone as 10574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10574-1-AP (same clone as 10574-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 10574-1-AP (same clone as 10574-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10574-1-AP (same clone as 10574-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10574-1-AP (same clone as 10574-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10574-1-PBS cible SUV39H1 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SUV39H1 Protéine recombinante Ag0854
• Nom complet: suppressor of variegation 3-9 homolog 1 (Drosophila)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC006238
• Symbole du gène: SUV39H1
• Identification du gène (NCBI): 6839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.

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