Anticorps Recombinant de lapin anti-SUZ12

SUZ12 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242491B3

N° de cat : 84895-3-PBS

Synonymes

KIAA0160, Joined to JAZF1 protein, JJAZ1, CHET9, ChET 9 protein

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Galerie de données de validation

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  WB analysis using 84895-3-RR (same clone as 84895-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84895-3-RR (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84895-3-RR (same clone as 84895-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84895-3-RR (same clone as 84895-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84895-3-RR (same clone as 84895-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUZ12 Recombinant antibody (84895-3-RR, Clone:242491B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84895-3-PBS in a different storage buffer formulation.

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Informations sur le produit

84895-3-PBS cible SUZ12 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: suppressor of zeste 12 homolog (Drosophila)
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: NM_015355
• Symbole du gène: SUZ12
• Identification du gène (NCBI): 23512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

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