Anticorps Polyclonal de lapin anti-SWAP70

SWAP70 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31042-1-AP

Synonymes

FLJ39540, HSPC321, KIAA0640, SWAP 70, SWAP 70 protein, SWAP70, Switch associated protein 70

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Galerie de données de validation

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  WB analysis using 31042-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31042-1-AP (SWAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31042-1-AP cible SWAP70 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SWAP70 Protéine recombinante Ag34855
• Nom complet: SWAP-70 protein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC000616
• Symbole du gène: SWAP70
• Identification du gène (NCBI): 23075
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SWAP70 (switching B cell complex subunit), also known as HSPC321. It is expected to be located in cell membrane, cytoplasm and nucleus, the protein is mainly expressed in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes, macrophages and mature B-cells (PMID: 10681448). The calculated molecular weight of SWAP70 is 69 kDa. SWAP-70 is a component of an enzyme complex that recombines Ig switch regions in vitro. It is reported that the cloning of the human cDNA and its B lymphocyte-specific expression. Although its sequence contains three nuclear localization signals, in small resting B cells, SWAP-70 is mainly found in the cytoplasm. On stimulation, SWAP-70 translocates to the nucleus. In activated, class-switching B cell cultures, it is associated with membrane IgG, but not IgM. The membrane Ig association requires a functional pleckstrin homology domain and is controlled by the C terminus. It is suggested that SWAP-70 is involved not only in nuclear events but also in signaling in B cell activation (PMID: 10681448).