Anticorps Monoclonal anti-SYK

• Phare
• Validé par KD/KO

SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

4C4A12

N° de cat : 66721-1-Ig

Synonymes

4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase

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Galerie de données de validation

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  WB analysis using 66721-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66721-1-Ig (SYK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66721-1-Ig

  WB result of SYK antibody (66721-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells.

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  WB analysis of THP-1 using 66721-1-Ig

  THP-1 (WT and SYK KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 66721-1-Ig incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of MDA-MB-231 using 66721-1-Ig

  MDA-MB-231 lysates prepared and IP of SYK performed using 2.0 μg of 66721-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human tonsillitis using 66721-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66721-1-Ig (SYK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66721-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66721-1-Ig (SYK antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules Ramos, cellules THP-1
• Résultats positifs en IP: cellules MDA-MB-231,
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

66721-1-Ig cible SYK dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SYK Protéine recombinante Ag17654
• Nom complet: spleen tyrosine kinase
• Masse moléculaire calculée: 635 aa, 72 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001645
• Symbole du gène: SYK
• Identification du gène (NCBI): 6850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Publications

Species	Application	Title
mouse	WB	Cancer Lett; SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.; Authors - Tian-Jiao Li
human	WB	Exp Cell Res; CD93 promotes acute myeloid leukemia development and is a potential therapeutic target; Authors - Jie Jia
mouse	WB,IF	Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian
mouse	WB	Cancer Cell; Distinct roles of TREM2 in central nervous system cancers and peripheral cancers; Authors - Jian Zhong
human	WB,IP,IF	F1000Res; Identification of high-performing antibodies for tyrosine-protein kinase SYK for use in Western Blot, immunoprecipitation and immunofluorescence; Authors - Walaa Alshafie ; KO Validated
pig,mouse	WB	PLoS Pathog; Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation; Authors - Xiaohua Zhang

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