Anticorps Monoclonal anti-SYK

• Phare
• Validé par KD/KO

SYK Monoclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

4C4A12

N° de cat : 66721-1-PBS

Synonymes

4C4A12, EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase

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Galerie de données de validation

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  WB analysis using 66721-1-Ig (same clone as 66721-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66721-1-Ig (SYK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66721-1-Ig (same clone as 66721-1-PBS)

  WB result of SYK antibody (66721-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SYK transfected Raji cells. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66721-1-Ig (same clone as 66721-1-PBS)

  THP-1 (WT and SYK KO) lysates prepared with RIPA buffer, 30 μg protein loaded. 66721-1-Ig incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IP experiment of MDA-MB-231 using 66721-1-Ig (same clone as 66721-1-PBS)

  MDA-MB-231 lysates prepared and IP of SYK performed using 2.0 μg of 66721-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66721-1-Ig (SYK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66721-1-Ig (same clone as 66721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66721-1-Ig (SYK antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66721-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66721-1-PBS cible SYK dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SYK Protéine recombinante Ag17654
• Nom complet: spleen tyrosine kinase
• Masse moléculaire calculée: 635 aa, 72 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001645
• Symbole du gène: SYK
• Identification du gène (NCBI): 6850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

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