Anticorps Polyclonal de lapin anti-Symplekin

Symplekin Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 11519-1-AP

Synonymes

SPK, SYM, SYMPK, symplekin

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Galerie de données de validation

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  WB analysis of K-562 using 11519-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11519-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11519-1-AP

  IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 11519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du côlon humain, tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11519-1-AP cible Symplekin dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Symplekin Protéine recombinante Ag2081
• Nom complet: symplekin
• Masse moléculaire calculée: 1274 aa, 141 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC006567
• Symbole du gène: Symplekin
• Identification du gène (NCBI): 8189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol; SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells; Authors - Rui Wu ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Detects endogenous protein with high sensitivity in Western blot both in HeLa and Jurkat cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat, HeLa

Symplekin Antibody Western Blot validation (1:1000 dilution) in Jurkat, HeLa (Cat no:11519-1-AP)

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