Anticorps Polyclonal de lapin anti-SYN1-Specific

SYN1-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

36

Avis (2)

N° de cat : 20258-1-AP

Synonymes

Brain protein 4.1, SYN1, SYN1a, SYN1b, SYN1-Specific, Synapsin 1, synapsin I, SYNI

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Galerie de données de validation

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  WB analysis of mouse brain using 20258-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20258-1-AP (SYN1-Specific Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20258-1-AP

  IP Result of anti-SYN1-Specific (IP:20258-1-AP, 4ug; Detection:20258-1-AP 1:3000) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20258-1-AP (SYN1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Informations sur le produit

20258-1-AP cible SYN1-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synapsin I
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_006950
• Symbole du gène: SYN1
• Identification du gène (NCBI): 6853
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYN1, also named as Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).

Publications

Species	Application	Title
mouse	WB	Environ Health Perspect; Evaluation of Neurotoxicity in BALB/c Mice following Chronic Exposure to Polystyrene Microplastics; Authors - Haibo Jin
rat	WB	Neurobiol Stress; Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.; Authors - Zhuoyu Ren
human	WB,IF	Prog Neurobiol; Islet Amyloid Polypeptide Triggers α-synuclein Pathology in Parkinson's Disease; Authors - Lanxia Meng
mouse	WB	Brain Behav Immun; Hippocampal alpha-synuclein mediates depressive-like behaviors.; Authors - Tingfu Du
mouse	WB	Sci Total Environ; Manganese-induced α-synuclein overexpression promotes the accumulation of dysfunctional synaptic vesicles and hippocampal synaptotoxicity by suppressing Rab26-dependent autophagy in presynaptic neurons; Authors - Zhuo Ma
mouse	WB	ACS Chem Neurosci; Merazin Hydrate Produces Rapid Antidepressant Effects Depending on Activating mTOR Signaling by Upregulating Downstream Synaptic Proteins in the Hippocampus.; Authors - Chao Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical cells went trough ICC and the SYN1 antibody at the 1/50 dilution and MAP2 antibody at the 1/1000 dilution were used overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: Human primary cortical cells

SYN1-Specific Antibody Immunohistochemistry validation (1/50 dilution) in Human primary cortical cells (Cat no:20258-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain