Anticorps Polyclonal de lapin anti-Syncoilin

Syncoilin Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 25151-1-AP

Synonymes

SYNC, Syncoilin-1, Syncoilin 1, SYNC1

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Galerie de données de validation

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  WB analysis using 25151-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25151-1-AP (Syncoilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 25151-1-AP

  IP result of anti-Syncoilin (IP:25151-1-AP, 4ug; Detection:25151-1-AP 1:800) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25151-1-AP (Syncoilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25151-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25151-1-AP (SYNC Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris,
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

25151-1-AP cible Syncoilin dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Syncoilin Protéine recombinante Ag18565
• Nom complet: syncoilin, intermediate filament protein
• Masse moléculaire calculée: 482 aa, 55 kDa
• Poids moléculaire observé: 64-68 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC119700
• Symbole du gène: SYNC
• Identification du gène (NCBI): 81493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Syncoilin also known as SYNC1, is a member of the intermediate filament family with an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. Syncoilin is highly expressed in skeletal and cardiac muscle. In normal skeletal muscle, syncoilin is concentrated at the neuromuscular junction(PMID: 11053421). Western blot analysis detected 64 kDa and 55 kDa syncoilin proteins in mouse heart(PMID: 11053421).

Publications

Species	Application	Title
mouse	IF	Invest Ophthalmol Vis Sci; The Cytoskeleton in the Extraocular Muscles of Desmin Knockout Mice.; Authors - Maria Angels Rodríguez

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (03-31-2022)

Strong band around predicted size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

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