Anticorps Recombinant de lapin anti-SYNJ2BP

SYNJ2BP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250205D7

N° de cat : 85870-4-RR

Synonymes

ARIP2, OMP25, Synaptojanin-2-binding protein

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Galerie de données de validation

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  WB analysis of rat kidney using 85870-4-RR

  Rat kidney tissue lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85870-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85870-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARIP2; SYNJ2BP antibody (85870-4-RR, Clone: 250205D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85870-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85870-4-RR against Human SYNJ2BP were performed. The affinity constant is 0.68 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules SGC-7901, cellules SH-SY5Y, tissu cardiaque humain, tissu rénal de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85870-4-RR cible SYNJ2BP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SYNJ2BP Protéine recombinante Ag8130
• Nom complet: synaptojanin 2 binding protein
• Masse moléculaire calculée: 145 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC007704
• Symbole du gène: SYNJ2BP
• Identification du gène (NCBI): 55333
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

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