Anticorps Recombinant de lapin anti-SYNPO

SYNPO Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4K5

N° de cat : 80721-1-PBS

Synonymes

Synaptopodin, 4K5, KIAA1029, snaptopodin

---

Galerie de données de validation

• 

  WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 80721-1-RR (same clone as 80721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80721-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

---

Informations sur le produit

80721-1-PBS cible SYNPO dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SYNPO Protéine recombinante Ag31287
• Nom complet: synaptopodin
• Masse moléculaire calculée: 929 aa, 99 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC146665
• Symbole du gène: SYNPO
• Identification du gène (NCBI): 11346
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

{{ptg:RelatedPrimaryAntibodies}}