Anticorps Polyclonal de lapin anti-Synaptophysin

Synaptophysin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

146

Avis (2)

N° de cat : 17785-1-AP

Synonymes

SYN, synaptophysin, SYP

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Galerie de données de validation

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  WB analysis using 17785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17785-1-AP (Synaptophysin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17785-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17785-1-AP (SYP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 17785-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17785-1-AP (SYP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17785-1-AP

  IP Result of anti-Synaptophysin; SYP (IP:17785-1-AP, 3ug; Detection:17785-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17785-1-AP (Synaptophysin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pituitary adenoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pituitary adenoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of ganglioma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of ganglioma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded ganglioma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of neuroblastoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of neuroblastoma using 17785-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 17785-1-AP (Synaptophysin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse pancreas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptophysin antibody (17785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of PC-12 using 17785-1-AP

  Immunofluorescent analysis of PC-12 cells using 17785-1-AP (Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 17785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 17785-1-AP (Synaptophysin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pancréatique de souris, tissu cérébral de souris, tissu cérébral humain, tissu d'adénome hypophysaire humain, tissu de gangliome, tissu de neuroblastome, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique de souris, cellules PC-12, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de gliome humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 114 publications below
• IHC: See 13 publications below
• IF: See 37 publications below

Informations sur le produit

17785-1-AP cible Synaptophysin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Synaptophysin Protéine recombinante Ag11781
• Nom complet: synaptophysin
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: BC064550
• Symbole du gène: SYP
• Identification du gène (NCBI): 6855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
mouse, rat	WB,IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
mouse	WB,IF	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	IF	Mol Cell; Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.; Authors - Zhengxin Ying
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandra (Verified Customer) (06-11-2023)

Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: P19 derived neurons

Synaptophysin Antibody Immunofluorescence validation (1:300 dilution) in P19 derived neurons (Cat no:17785-1-AP)

FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human primary cortical neurons

Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)

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