Anticorps Recombinant de lapin anti-Synaptotagmin-14

Synaptotagmin-14 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240866C2

N° de cat : 83923-1-PBS

Synonymes

SytXIV, SYT14, Synaptotagmin XIV, 240866C2

---

Galerie de données de validation

• 

  WB analysis using 83923-1-RR (same clone as 83923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83923-1-RR (SYT14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 83923-1-RR (same clone as 83923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83923-1-RR (Synaptotagmin-14 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83923-1-RR (same clone as 83923-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Synaptotagmin-14 Recombinant antibody (83923-1-RR, Clone:240866C2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83923-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

83923-1-PBS cible Synaptotagmin-14 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synaptotagmin XIV
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_153262
• Symbole du gène: Synaptotagmin-14
• Identification du gène (NCBI): 255928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).

{{ptg:RelatedPrimaryAntibodies}}