Anticorps Polyclonal de lapin anti-SYT17

• Phare
• Validé par KD/KO

SYT17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15413-1-AP

Synonymes

synaptotagminXVII, Synaptotagmin-17, Synaptotagmin17, Synaptotagmin XVII, Synaptotagmin 17

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Galerie de données de validation

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  WB analysis of human kidney using 15413-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15413-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15413-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15413-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 15413-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15413-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15413-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15413-1-AP (SYT17 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15413-1-AP

  IP result of anti-SYT17 (IP:15413-1-AP, 4ug; Detection:15413-1-AP 1:800) with mouse brain tissue lysate 6000ug.

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  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 15413-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15413-1-AP (SYT17 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15413-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYT17 antibody (15413-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu rénal humain, cellules A375, cellules PC-3, tissu cérébral de souris, tissu cérébral humain, tissu pulmonaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15413-1-AP cible SYT17 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SYT17 Protéine recombinante Ag7691
• Nom complet: synaptotagmin XVII
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC004518
• Symbole du gène: SYT17
• Identification du gène (NCBI): 51760
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYT17, or synaptotagmin 17, is a protein that belongs to the synaptotagmin family, which is known for its role in regulating exocytosis at the plasma membrane. Reports find high levels of expression in hippocampus, particularly in pyramidal cell layers and in cultured hippocampal neurons. SYT17 is thus a multifunctional regulator of intracellular protein trafficking in excitatory hippocampal neurons, modulating both neural development and synaptic physiology. (PMID: 31387992, PMID: 36332665)

Publications

Species	Application	Title
human	IHC,IF	Int Immunol; Increased urinary exosomal SYT17 levels in chronic active antibody-mediated rejection after kidney transplantation via the IL-6 amplifier.; Authors - Yusuke Takada ; KD Validated

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