Anticorps Polyclonal de lapin anti-SYT7

SYT7 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 19741-1-AP

Synonymes

IPCA 7, IPCA-7, PCANAP7, Prostate cancer-associated protein 7, Synaptotagmin 7

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Galerie de données de validation

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  WB analysis using 19741-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19741-1-AP (SYT7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19741-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19741-1-AP (SYT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 19741-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT7 antibody (19741-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

19741-1-AP cible SYT7 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: synaptotagmin VII
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: NM_004200
• Symbole du gène: SYT7
• Identification du gène (NCBI): 9066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYT7, also named as PCANAP7 and IPCA-7, belongs to the synaptotagmin family. SYT7 may be involved in Ca²⁺-dependent exocytosis of secretory vesicles through Ca²⁺ and phospholipid binding to the C2 domain or may serve as Ca²⁺ sensors in the process of vesicular trafficking and exocytosis.

Publications

Species	Application	Title
mouse	IF	Brain Res Bull; Double-strand-break repair protein rad21 homolog/Synaptotagmin-7 alleviates Alzheimer's disease in mice by promoting M2 polarization of microglia; Authors - Cheng Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olga (Verified Customer) (01-17-2025)

I was disapointed as it did not recognize the band of declared 42-46 kDa in any tissue and cell lysate tested - only the bands of about 55 kDa and mouse brain tissue and cells - and a band of about 100 kDa in all lanes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (forebrain, liver, lung,heart)

SYT7 Antibody Western Blot validation (1:1000 dilution) in HEK293T, Neuro2A, mouse primary cerebellar neurons, mouse tissues (forebrain, liver, lung,heart) (Cat no:19741-1-AP)

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