Anticorps Recombinant de lapin anti-SYTL5

SYTL5 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242838D12

N° de cat : 85108-5-PBS

Synonymes

242838D12, SLP5, synaptotagmin like 5, Synaptotagmin like protein 5, Synaptotagmin-like protein 5

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Galerie de données de validation

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  WB analysis of SK-N-SH using 85108-5-RR (same clone as 85108-5-PBS)

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 85108-5-RR (same clone as 85108-5-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 85108-5-RR (SYTL5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 85108-5-RR (SYTL5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 85108-5-RR (same clone as 85108-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using SYTL5 antibody (85108-5-RR, Clone: 242838D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85108-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85108-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85108-5-RR against Human SYTL5 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85108-5-PBS cible SYTL5 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SYTL5 Protéine recombinante Ag21698
• Nom complet: synaptotagmin-like 5
• Masse moléculaire calculée: 730 aa, 81 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC131585
• Symbole du gène: SYTL5
• Identification du gène (NCBI): 94122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SYTL5, also known as SLP5, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by the presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL5 promotes the development and progression of papillary thyroid carcinoma (PTC) by activating the NF-κB signaling pathway (PMID: 36378561).

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