Anticorps Polyclonal de lapin anti-HRD1/SYVN1

• Phare
• Validé par KD/KO

HRD1/SYVN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP-MS, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

50

N° de cat : 13473-1-AP

Synonymes

HRD1, HRD1/SYVN1, KIAA1810, Synovial apoptosis inhibitor 1, SYVN1

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Galerie de données de validation

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  WB analysis using 13473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat stomach using 13473-1-AP

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13473-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13473-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 13473-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 13473-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13473-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13473-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13473-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 13473-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13473-1-AP (SYVN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13473-1-AP

  IP Result of anti-SYVN1 (IP:13473-1-AP, 4ug; Detection:13473-1-AP 1:1000) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human kidney using 13473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13473-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13473-1-AP (SYVN1 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13473-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13473-1-AP (SYVN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MDA-MB-453s, cellules NIH/3T3, cellules Raji, tissu d'estomac de rat, tissu d'estomac de souris, tissu ovarien de souris, tissu pancréatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 48 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below

Informations sur le produit

13473-1-AP cible HRD1/SYVN1 dans les applications de WB, IP-MS, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HRD1/SYVN1 Protéine recombinante Ag4267
• Nom complet: synovial apoptosis inhibitor 1, synoviolin
• Masse moléculaire calculée: 617 aa, 68 kDa
• Poids moléculaire observé: 68-76 kDa
• Numéro d’acquisition GenBank: BC030530
• Symbole du gène: SYVN1
• Identification du gène (NCBI): 84447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	IP,WB	J Clin Invest; Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders; Authors - Huilun Helen Wang
human	WB,IF,IP,IP-MS	Mol Cancer; CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.; Authors - Ya Fan
human	WB	Mol Cell; Redundant and Antagonistic Roles of XTP3B and OS9 in Decoding Glycan and Non-glycan Degrons in ER-Associated Degradation.; Authors - Annemieke T van der Goot ; KD Validated
		Mol Cell; Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.; Authors - Corey N Cunningham
human	WB	Nat Commun; PGRMC1 acts as a size-selective cargo receptor to drive ER-phagic clearance of mutant prohormones.; Authors - Yu-Jie Chen