Anticorps Polyclonal de lapin anti-Sam50

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 28679-1-AP

Synonymes

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

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Galerie de données de validation

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  WB analysis using 28679-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 28679-1-AP (SAMM50 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28679-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28679-1-AP (Sam50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (28679-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 28679-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28679-1-AP (Sam50 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

28679-1-AP cible Sam50 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Sam50 Protéine recombinante Ag29152
• Nom complet: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007830
• Symbole du gène: Sam50
• Identification du gène (NCBI): 25813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Publications

Species	Application	Title
human,mouse	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	IF	Front Cardiovasc Med; Samm50 Promotes Hypertrophy by Regulating Pink1-Dependent Mitophagy Signaling in Neonatal Cardiomyocytes; Authors - Ran Xu
mouse	WB	Int J Biol Sci; Loss of MTX2 causes mitochondrial dysfunction, podocyte injury, nephrotic proteinuria and glomerulopathy in mice and patients; Authors - Ting Li
mouse,rat	WB,IF,IP	Cell Signal; Mitochondrial outer membrane protein Samm50 protects against hypoxia-induced cardiac injury by interacting with Shmt2; Authors - Yufei Zhou
human	WB	Cancer Res Commun; N-Myristoyltransferase Inhibition causes Mitochondrial Iron Overload and Parthanatos in TIM17A-dependent Aggressive Lung Carcinoma; Authors - Sofia Geroyska
human	WB	J Cell Sci; Regulation of mitochondrial cristae organization by Myo19, Miro1/2 and Metaxin 3; Authors - Samruddhi S Shembekar

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