Anticorps Monoclonal anti-Sam50

Sam50 Monoclonal Antibody for IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H6H9

N° de cat : 67425-1-PBS

Synonymes

SAMM50, 2H6H9, CGI 51, CGI-51, OMP85

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Galerie de données de validation

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  IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67425-1-Ig (same clone as 67425-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67425-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67425-1-PBS cible Sam50 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Sam50 Protéine recombinante Ag15155
• Nom complet: sorting and assembly machinery component 50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC007830
• Symbole du gène: Sam50
• Identification du gène (NCBI): 25813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

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