Anticorps Polyclonal de lapin anti-Securin

• Phare
• Validé par KD/KO

Securin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18040-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 18040-1-AP (same clone as 18040-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 18040-1-AP (same clone as 18040-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18040-1-AP (same clone as 18040-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 18040-1-AP (same clone as 18040-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18040-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

18040-1-PBS cible Securin dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Securin Protéine recombinante Ag12670
• Nom complet: pituitary tumor-transforming 1
• Masse moléculaire calculée: 202 aa, 22 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC101834
• Symbole du gène: Securin
• Identification du gène (NCBI): 9232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

{{ptg:RelatedPrimaryAntibodies}}