Anticorps Recombinant de lapin anti-P-Selectin/CD62P

P-Selectin/CD62P Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240766D3

N° de cat : 83947-5-RR

Synonymes

P-selectin, CD62P, 240766D3, PADGEM, P selectin

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Galerie de données de validation

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  WB analysis of human peripheral blood leukocyte using 83947-5-RR

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 83947-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 83947-5-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 83947-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83947-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 83947-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83947-5-RR (P-Selectin/CD62P antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 83947-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 83947-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using P-Selectin/CD62P antibody (83947-5-RR, Clone: 240766D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83947-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83947-5-RR against Mouse P-Selectin/CD62P were performed. The affinity constant is 7.97 nM.

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Applications testées

• Résultats positifs en WB: leucocyte de sang périphérique humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83947-5-RR cible P-Selectin/CD62P dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: selectin, platelet
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_011347.2
• Symbole du gène: Selp
• Identification du gène (NCBI): 20344
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

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