Anticorps Polyclonal de lapin anti-Septin 5

• Phare
• Validé par KD/KO

Septin 5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11631-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 11631-1-AP (same clone as 11631-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  WB analysis using 11631-1-AP (same clone as 11631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 11631-1-AP (same clone as 11631-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11631-1-AP (same clone as 11631-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (Septin 5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11631-1-AP (same clone as 11631-1-PBS)

  IP result of anti-Septin 5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug. This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11631-1-AP (same clone as 11631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11631-1-AP (Septin 5 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11631-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11631-1-PBS cible Septin 5 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 5 Protéine recombinante Ag2205
• Nom complet: septin 5
• Masse moléculaire calculée: 369 aa, 43 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC025261
• Symbole du gène: Septin 5
• Identification du gène (NCBI): 5413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Septin 5, a septin family member formerly called cell division control related protein 1 (CDCrel), is encoded in the 200 kb region and is abundantly expressed in the developing and adult brains of mice and humans. Although its constitutive deletion does not cause detectible structural or developmental abnormalities in the mouse brain, Septin 5 has been implicated in subtle neuronal and behavioral functions. Septin 5 is expressed in presynaptic terminals and exerts negative regulatory control over neurotransmitter release by binding to SNARE-protein, which has been implicated in neuropsychiatric disorders (PMID: 19240081). Septin 5 has 2 isoforms with the molecular mass of 39 and 43 kDa.

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