Anticorps Monoclonal anti-Septin 8

Septin 8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A12G9

N° de cat : 66188-1-PBS

Synonymes

SEPT8, KIAA0202, SEP2, SEPTIN8, Septin-8

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Galerie de données de validation

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  WB analysis of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66188-1-Ig (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66188-1-Ig (Septin 8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66188-1-Ig (same clone as 66188-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 8 antibody (66188-1-Ig, Clone: 4A12G9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66188-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66188-1-PBS cible Septin 8 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Septin 8 Protéine recombinante Ag19114
• Nom complet: septin 8
• Masse moléculaire calculée: 55.6 kDa
• Poids moléculaire observé: 50-58 kDa
• Numéro d’acquisition GenBank: BC001329
• Symbole du gène: Septin 8
• Identification du gène (NCBI): 23176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Chromatographie d'affinité thiophilique
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are membrane-associated GTPases which are involved in cytokinesis and cellular morphogenesis. Septin 8 is an interaction partner of Septin 4. It may play a role in platelet secretion.

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