Anticorps Polyclonal de lapin anti-Serotonin transporter

• Phare
• Validé par KD/KO

Serotonin transporter Polyclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19559-1-PBS

Synonymes

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT

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Galerie de données de validation

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  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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  WB analysis using 19559-1-AP (same clone as 19559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 19559-1-AP (same clone as 19559-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19559-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19559-1-PBS cible Serotonin transporter dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa and 140 kDa
• Numéro d’acquisition GenBank: NM_001045
• Symbole du gène: Serotonin transporter
• Identification du gène (NCBI): 6532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).

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