Anticorps Recombinant de lapin anti-Serpin B3/4

Serpin B3/4 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250057B3

N° de cat : 85807-2-RR

Synonymes

SERPINB3, SERPINB3/4, Protein T4 A, Protein T4-A, SCCA-1

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Galerie de données de validation

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  WB analysis using 85807-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85807-2-RR (SERPINB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85807-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85807-2-RR against Human Serpin B3/4 were performed. The affinity constant is 0.95 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HaCaT, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85807-2-RR cible Serpin B3/4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Serpin B3/4 Protéine recombinante Ag24846
• Nom complet: serpin peptidase inhibitor, clade B (ovalbumin), member 3
• Masse moléculaire calculée: 390 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC005224
• Symbole du gène: SERPINB3
• Identification du gène (NCBI): 6317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.

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