Anticorps Monoclonal anti-Serpin C1/Antithrombin III

Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B2E5

N° de cat : 66052-1-PBS

Synonymes

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, Antithrombin-III

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Galerie de données de validation

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  WB analysis of human plasma using 66052-1-Ig (same clone as 66052-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66052-1-Ig (same clone as 66052-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66052-1-Ig (same clone as 66052-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66052-1-Ig (same clone as 66052-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

• 

  IF Staining of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66052-1-PBS cible Serpin C1/Antithrombin III dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Serpin C1/Antithrombin III Protéine recombinante Ag9880
• Nom complet: serpin peptidase inhibitor, clade C (antithrombin), member 1
• Masse moléculaire calculée: 464 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC022309
• Symbole du gène: Serpin C1/Antithrombin III
• Identification du gène (NCBI): 462
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

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