Anticorps Monoclonal anti-Serum amyloid P component

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1D10

N° de cat : 66084-1-PBS

Synonymes

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

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Galerie de données de validation

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  WB analysis of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66084-1-Ig (same clone as 66084-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66084-1-PBS cible Serum amyloid P component dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Serum amyloid P component Protéine recombinante Ag14957
• Nom complet: amyloid P component, serum
• Masse moléculaire calculée: 223 aa, 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC007058
• Symbole du gène: Serum amyloid P component
• Identification du gène (NCBI): 325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

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