Anticorps Monoclonal anti-Sestrin 2

• Phare
• Validé par KD/KO

Sestrin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1A4

N° de cat : 66297-1-PBS

Synonymes

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2

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Galerie de données de validation

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  WB analysis of HEK-293T using 66297-1-Ig (same clone as 66297-1-PBS)

  WB result of Sestrin 2 antibody (66297-1-Ig, 1:500) with si-control and si-Sestrin 2 transfected Hek293 cells. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66297-1-Ig (same clone as 66297-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66297-1-Ig (same clone as 66297-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66297-1-Ig (same clone as 66297-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66297-1-Ig (same clone as 66297-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66297-1-Ig (Sestrin2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66297-1-Ig (same clone as 66297-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Sestrin 2 antibody (66297-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66297-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66297-1-PBS cible Sestrin 2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Sestrin 2 Protéine recombinante Ag20638
• Nom complet: sestrin 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC013304
• Symbole du gène: SESN2/Sestrin 2
• Identification du gène (NCBI): 83667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

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