Anticorps Recombinant de lapin anti-Sestrin 2

Sestrin 2 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240034H3

N° de cat : 83202-3-PBS

Synonymes

SESN2, Sestrin2, 240034H3, EC:1.11.1.-, Hi95

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Galerie de données de validation

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  WB analysis using 83202-3-RR (same clone as 83202-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83202-3-RR (Sestrin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83202-3-RR (same clone as 83202-3-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Sestrin 2 Recombinant antibody (83202-3-RR, Clone:240034H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83202-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00278-1

  Cytometric bead array standard curve of MP00278-1, Sestrin 2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83202-2-PBS. Detection antibody: 83202-3-PBS. Standard: Ag1247. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83202-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83202-3-RR against Human Sestrin 2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83202-3-PBS cible Sestrin 2 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Sestrin 2 Protéine recombinante Ag1247
• Nom complet: sestrin 2
• Masse moléculaire calculée: 54 kDa
• Numéro d’acquisition GenBank: BC013304
• Symbole du gène: SESN2/Sestrin 2
• Identification du gène (NCBI): 83667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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