Anticorps Recombinant de lapin anti-Siglec-7/CD328

Siglec-7/CD328 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241525H1

N° de cat : 98183-1-PBS

Synonymes

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98183-1-RR (same clone as 98183-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone: 241525H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were not fixed. This data was developed using the same antibody clone with 98183-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98183-1-RR (same clone as 98183-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone:241525H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98183-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98183-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98183-1-RR against Human Siglec-7/CD328 were performed. The affinity constant is 0.22 nM.

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Informations sur le produit

98183-1-PBS cible Siglec-7/CD328 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sialic acid binding Ig-like lectin 7
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: NM_014385.4
• Symbole du gène: Siglec-7
• Identification du gène (NCBI): 27036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

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