Anticorps Recombinant de lapin anti-Siglec-7/CD328

Siglec-7/CD328 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241525H1

N° de cat : 98183-1-RR

Synonymes

SIGLEC7, Siglec-7, D siglec, CD328, AIRM-1

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98183-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone: 241525H1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98183-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-7/CD328 Rabbit Recombinant Antibody (98183-1-RR, Clone:241525H1) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98183-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98183-1-RR against Human Siglec-7/CD328 were performed. The affinity constant is 0.22 nM.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98183-1-RR cible Siglec-7/CD328 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sialic acid binding Ig-like lectin 7
• Masse moléculaire calculée: 51 kDa
• Numéro d’acquisition GenBank: NM_014385.4
• Symbole du gène: Siglec-7
• Identification du gène (NCBI): 27036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

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