Anticorps Recombinant de lapin anti-Siglec-8

Siglec-8 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

241275A5

N° de cat : FITC-98177

Synonymes

SIGLEC8, SIGLEC 8, Sialoadhesin family member 2, Sialic acid-binding Ig-like lectin 8, SAF2

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using FITC-98177

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human Siglec-8 Rabbit RecAb (FITC-98177, Clone: 241275A5) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9), and 5 ul CoraLite® Plus 647 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (CL647-65612, Clone: 3G8). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using FITC-98177

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human Siglec-8 Rabbit RecAb (FITC-98177, Clone: 241275A5) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98177 cible Siglec-8 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sialic acid binding Ig-like lectin 8
• Masse moléculaire calculée: 499 aa, 54 kDa
• Numéro d’acquisition GenBank: BC053319
• Symbole du gène: SIGLEC8
• Identification du gène (NCBI): 27181
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).

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