Anticorps Recombinant de lapin anti-Siglec-9

Siglec-9 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241799C10

N° de cat : 98264-6-RR

Synonymes

SIGLEC9, 241799C10, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98264-6-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98264-6-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98264-6-RR

  Biolayer interferometry (BLl) kinetic assays of 98264-6-RR against Human Siglec-9 were performed. The affinity constant is 0.682 nM.

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Applications testées

• Résultats positifs en cytométrie: human peripheral blood leukocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98264-6-RR cible Siglec-9 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Siglec-9 Protéine recombinante Eg1084
• Nom complet: sialic acid binding Ig-like lectin 9
• Masse moléculaire calculée: 50 kDa
• Numéro d’acquisition GenBank: NM_014441.3
• Symbole du gène: Siglec-9
• Identification du gène (NCBI): 27180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

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